primer-design
There are 6 repositories under primer-design topic.
- polyLanguage:Go 653
billzt / PrimerServer2
PrimerServer2: a high-throughput primer design and specificity-checking platform
Language:Python 69widdowquinn / find_differential_primers
Code for design of diagnostic PCR primers, and metabarcoding markers.
Language:Python 56gear-genomics / dicey
In-silico PCR, primer design and padlock design for in-situ sequencing
Language:C++ 41biologger / speciesprimer
The SpeciesPrimer pipeline is intended to help researchers finding specific primer pairs for the detection and quantification of bacterial species in complex ecosystems.
Language:Python 36Edinburgh-Genome-Foundry / Primavera
:cherry_blossom: Python library for primer-based verification of DNA assemblies: primer selection, data analyis, etc.
Language:Python 33VascoElbrecht / PrimerMiner
R mased batch sequence downloader, with primer development and in silico evaluation capabilities
Language:R 30github / mlops
Use GitHub to facilitate automation, collaboration and reproducibility in your machine learning workflows.
Language:JavaScript 23dieterich-lab / circtools
circtools: a modular, python-based framework for circRNA-related tools that unifies several functionalities in a single, command line driven software.
Language:Python 22jonas-fuchs / varVAMP
Design degenerated primers on highly variable alignments for full genome sequencing or qPCR. Specifically developed for viruses.
Language:Python 19- Language:CSS 14
Obsolete!! Begin to turn to PrimerServer2
Language:Perl 10In-silico PCR amplification on complete genomes
Language:JavaScript 9smitbarmase / pods
🚀 Project management and communication tool for teams.
Language:TypeScript 9- primerdriver
kvdomingo / primerdriver
PrimerDriver - Automated design of mutagenic PCR primers
Language:Python 8 Owaiskhan9654 / Gene-Sequence-Primer-
Gene and Primer Sequence Analysis for SARS-CoV-2, EGFR(Non Small Lung Cancer Cell), Influenza DNAs ### How can I check my Oligo primers to ensure there are no significant primer design issues? - The difference between melting temperatures (Tm) of the primers should be less than 5°C. - The GC content should be between 35-80% or equivalent to the product being amplified. - The Delta G value of any self-dimers, hairpins, and heterodimers should be weaker (more positive) than -9.0 kcal/mole. Positive numbers indicate that the actual secondary structure shown will not form at all. - Avoid 3' complementarity between the two primers to prevent primer dimers. The IDT OligoAnalyzer APIs can be used to assess these different criteria for a proposed oligo. #### [Reference](https://sg.idtdna.com/pages/support/faqs/how-can-i-check-my-pcr-primers-using-the-oligoanalyzer-program-to-ensure-there-are-no-significant-primer-design-issues-)
Language:Jupyter Notebook 7rdpstaff / EcoFunPrimer
primer design pipeline
Language:Java 7ipb-halle / GoldenMutagenesis
Support for rapid design of primers for amino acid exchanges and saturation mutagenesis by Golden Gate cloning.
Language:R 4- Language:C++ 4
DanielSchuette / cloningPrimer
Web App, CLI, and API for designing cloning primer pairs - written in Go👩🏼🔬👨🏼🔬
Language:Go 3- Language:Python 3
Mohamed-Elmanzalawi / PCR-primer-script
Streamlining dataset analysis and primer design for efficient genetic studies. Our Project is driven by robust algorithms, aims to achieve quick and accurate results. Leveraging Muscle alignment and Primer-BLAST, the project simplifies the process to enhance the precision of genetic analysis.
Language:Python 3weinerlab / mtk_primer_generator
Automagically ✨ generate primers for Golden Gate cloning using the Mammalian Cloning Toolkit (MTK)
Language:Python 3sanjaynagi / AnoPrimer
Primer Design in Anopheles gambiae and funestus considering genetic variation
Language:Jupyter Notebook 2- Language:Python 1
- Language:R 1
- Language:R 1
eskutkaan-zz / biodata
a molecular solutions toolbox for molecular biologists.
Language:Python 1GEizaguirre / PolyPrimer
Tool in Perl for finding Polymorphisms in primers for human genes.
Language:Perl 1- Language:Python 1
leylabmpi / CoreGenomePrimers
Design clade-specific primers targeting a pan-genome core gene
Language:Jupyter Notebook 1swiing / hugo-theme-primer
Makes it easy to use github primer in hugo
Language:HTML 1alohadancemeow / mypost-but-github
👻 A mini social media inspired by Github, For learning how frontend+backend work together 🚀
Language:TypeScript 0padrong / TakaraGxl_Calculator
A calculator written in python 3 based off the takara GXL polymerase protocol. Used to calculate Tm.
Language:Python 0- Language:R 0
ShuXingYu94 / Primer-picker
Designing and blasting to find primer with specific PCR product
Language:Python 0
