There are 0 repository under gene-sequencing topic.
Compute over Data framework for public, transparent, and optionally verifiable computation
Gene and Primer Sequence Analysis for SARS-CoV-2, EGFR(Non Small Lung Cancer Cell), Influenza DNAs ### How can I check my Oligo primers to ensure there are no significant primer design issues? - The difference between melting temperatures (Tm) of the primers should be less than 5°C. - The GC content should be between 35-80% or equivalent to the product being amplified. - The Delta G value of any self-dimers, hairpins, and heterodimers should be weaker (more positive) than -9.0 kcal/mole. Positive numbers indicate that the actual secondary structure shown will not form at all. - Avoid 3' complementarity between the two primers to prevent primer dimers. The IDT OligoAnalyzer APIs can be used to assess these different criteria for a proposed oligo. #### [Reference](https://sg.idtdna.com/pages/support/faqs/how-can-i-check-my-pcr-primers-using-the-oligoanalyzer-program-to-ensure-there-are-no-significant-primer-design-issues-)
Compilation of Gene Analysis Algorithms and Mutation Search Algorithms for hopefully some useful Machine Learning application later.
Whitepaper on application of genetic alignment on religious discourses. Base corpus: LDS Journal of Discourses comprised of hundreds of religious discourses.
This project alligns genetic sequences locally
Perform a de-novo assembly of an unknown organism and report workflows and findings. Illumina PE 101x2, insert size 350bps +/- 50, PacBio long reads. Develop a Java program with a GUI to vizualise and report quality metrics for the best three assembly attempts.
Demo using Python/FastAPI/JS/jQuery/Bootstrap/ChartJS - (Backend + Frontend UI) framework with an input to add sequences, search for the 'G' Quadruplexes that displays the start & end positions in a table & a bar graph for better yield rate.
Gene download from NCBI database, sequence alignment and clusterization, realization of different bioinformatics algorithms within ITMO 4 sem course