There are 1 repository under confocal-microscopy topic.
Janelia Workstation
Simulation for sensorless adaptive optics (Confocal microscopy, Modal method)
ImageJ / Fiji macro to automatically export Leica LIF file as individual images (batch mode available)
🧪 Reproducing the concept of Confocal Laser Scanning Microscope. Using Arduino and easily found materials. Generating images in Grayscale just for fun.
Imaging experiments in Biology often involve the acquisition of confocal z-stack images to quantify fluorescent signal in a biological sample. These z-stacks are usually analyzed using open-source programs such as FIJI, which are notoriously frustrating to work with, and do not facility intuitive high-throughput analysis of many images at once. Manual image analysis is labor-intensive, time-consuming, and prone to human error. I compiled two notebooks to streamline two of the most commonly performed tasks in the analysis of confocal z-stack images: the generation of "pretty" maximum projections, and the analysis of pixel values/ fluorescence from sum projections.
Automated determination of cell membrane curvature from 3D stacks using the radius of osculating circle approach.
Képalkotás szenzor adatokból
Analysis performed for the Belyaeva et.al PNAS 2017 Paper
[Pillow] Denoising Images retrieved from Confocal Calcium Imaging.
Versatile Fiji-imagej macro for 2D quantification of each marker inside your immunofluorescence staining in less of 1 minute per image. Control critical steps for segmentation.
The MatLab code takes confocal TIFF images of cells as input and plots the distribution of different channels after segmentation of the image into features bases on the type of organelles. For getting the membrane features, distance transform is used to obtain a continuos membrane region.
Repository containing methods applied for the analysis of the data in the SERCA publication
Quantification of actin dynamics using difference maps (Vidali et al, 2010, The Plant Cell)
Analysis of actin dynamics using the method described by Higaki et al. (2009).
3D surface reconstruction from confocal microscopy stacks. This software package has been created for the anaylis of 3D vesicle shapes.