pommevilla / MetaFunPrimer

A qPCR primer design pipeline to target environmentally abundant functional genes

https://metafunprimer.readthedocs.io/en/latest/

pommevilla/MetaFunPrimer Issues

How install 'qsub'?
Closed 4 years ago1
Checking clustalo-1.2.4-Ubuntu-x86_64: Failed on a centos server.
Updated 4 months ago1
Docker
Updated a year ago1
Incorporate isPCR
Updated a year ago
Snakemake
Updated a year ago
Switch to GHA
Updated a year ago
mfpqpcr embedded get_pcr_product.py read primer files may trigger a bug
Updated 2 years ago
mfpdesign doesn't take arguments smoothly
Updated 3 years ago
Can the pipeline be run without qsub?
Updated 3 years ago
Primer design file prepared
Closed 4 years ago2
mfpcount output ...summmary.tsv error
Closed 4 years ago1
calc_sscore: change normalization technique
Closed 4 years ago1
Allow use of pipeline using only nucleotide file
Updated 4 years ago
get_pcr_product.py: allow 1 mismatch when evaluating primer amplification
Updated 4 years ago
Change call to python in count_presence and count_abundance
Closed 4 years ago
Add fna to searched for files
Closed 4 years ago
mfpqpcr outputs syntax error for empty input primer file
Updated 4 years ago
Unable to run job: denied: host
Closed 4 years ago
mfpdesign: adding additional EcoFunPrimer arguments
Updated 4 years ago
mfpcount: add threshold argument
Updated 4 years ago
Editing calls to parallel
Closed 5 years ago1
mfpprepare: general improvements
Updated 5 years ago
Documentation fixes
Updated 5 years ago
Combine count_presence and count_abundance
Updated 5 years ago
count_richness: Rename to count_presence
Closed 5 years ago1
job_header:
Updated 5 years ago
mfpcluster: Allow user to toggle between accurate and fast mode in CD-HIT
Updated 5 years ago
count_richness: add definition of richness
Closed 5 years ago1
mfpcluster: Add note about optimum clustering percentage
Closed 5 years ago1