pommevilla / MetaFunPrimer

A qPCR primer design pipeline to target environmentally abundant functional genes

Home Page:https://metafunprimer.readthedocs.io/en/latest/

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Allow use of pipeline using only nucleotide file

pommevilla opened this issue · comments

Pipeline currently requires a nucleotide file, a corresponding protein file, and a map file that shows which nucleotide sequence translates to which protein sequence.

Add arguments/options to the various parts of the pipeline to support the case where the user doesn't want to use the protein file.

Files affected:

  • mfpsearch
    • add an argument -m/--mode that takes in n or p to indicate whether to run in nucleotide or protein mode, respectively.
    • creation of the database and the search scripts will change depending on the mode used.
  • mfpprepare
    • add an argument n or p to indicate whether to run in nucleotide or protein mode, respectively.
    • if in nucleotide mode, should ignore the map argument.