A flask app that make selected pydna functionality available as a web service.
- The WebPCR simulator.
- Primer Tm calculator based on The Bio.SeqUtils.MeltingTemp module.
- Primer designer. Design two flanking primers, given a template sequence.
- Assembly primer designer. Design tailed primers for assembly by HR.
- Assembly simulator. Assemble a series of sequences by shared homology.
Installed on pythonanywhere like this:
mkvirtualenv --python=/usr/bin/python3.9 my-virtualenv
pip install --upgrade flask flask-wtf wtforms pydna
git clone https://github.com/BjornFJohansson/pydnaweb.git
Settings uder the "Web" tab in python anywhere:
Settings in the /var/www/pydna_pythonanywhere_com_wsgi.py file:
