hisplan / wdl-cellranger-gex

WDLized Cell Ranger GEX

wdl-cellranger-gex

WDLized Cell Ranger Gene Expression Pipeline

License

The pipeline code is available to everyone under the standard MIT license. However, the pipeline internally uses 10x software, so please make sure that you read and agree to 10x End User Software License.

Setup

The pipeline is a part of SCING (Single-Cell pIpeliNe Garden; pronounced as "sing" /siŋ/). For setup, please refer to this page. All the instructions below is given under the assumption that you have already configured SCING in your environment.

Create Job Files

You need two files for processing a GEX sample - one inputs file and one labels file. Use the following example files to help you create your configuration file:

• configs/template.inputs.json
• configs/template.labels.json

Reference

Use one of the URLs below for the reference genome:

Type	CellRangerGex.reference
GRCh38	https://cf.10xgenomics.com/supp/cell-exp/refdata-gex-GRCh38-2020-A.tar.gz
mm10	https://cf.10xgenomics.com/supp/cell-exp/refdata-gex-mm10-2020-A.tar.gz

Submit Your Job

conda activate scing

./submit.sh \
    -k ~/keys/cromwell-secrets.json \
    -i configs/your-sample.inputs.json \
    -l configs/your-sample.labels.json \
    -o CellRangerGex.options.aws.json