About default min read quality setup

[wuying1984]

Hi,

I am running NanoPlot to generate the QC matrix of my nanopore reads.
When I use the following command, I found that nanoplot uses the reads with quality over 5 to generate the report and figures. I wonder if I can setup some parameter so that nanoplot will not drop any reads and use all the reads to generate the report and figure. Thank you very much!
my command: nanoplot -t 12 -o output_folder --prefix sample_name -f png --fastq input.fastq.gz --plots kde

Best,
Ying

[Wouter De Coster]

NanoPlot by default uses all the reads. You may not have any reads with a quality below 5?

[Ying Wu]

Yes, I think so. Thank you very much!