GPL-244 As a R&D lead for quant hub (Scott Goodwin) I would like to remove the factor that QuantHub uses to convert CopyCount’s output into library concentrations so the ‘fudge factor’ can be changed in the lab without a software change (will be in the excel template)

[rl15]

The current version of QuantHub includes a fudge factor to reconcile what CopyCount puts out with what we used to get using the LC480’s internal analysis method on 10ul qPCRs. This factor isn’t wrong, but I want to move this fudging factor into the UKBB Excel pooling template downstream of QuantHub for a few different reasons:
a) The UKBB pooling template already incorporates a fudging factor to multiply everything which comes out of QuantHub by 1.5 (this was to reconcile what was coming out of the LC480 with the 10ul qPCR reactions with an even earlier method we used to use with a different qPCR machine); in the interests of transparency in the future it would be good to have all the fudging in one place in the process, if that makes sense?
b) There’s a possibility that it will transpire in future that the fudge factor might need to be tweaked when batches of qPCR reagents change (this hasn’t needed to happen so far when we have changed batches of reagents, but I can’t rule out that it won’t need to happen in the future); by taking this factor out of CopyCount we can deal with this without having to bother you guys about it