DPL-266 - Warning for tapestation quants where concentration is below 5nM (C=M, V=4)
stevieing opened this issue · comments
User story
The heron team will soon be swapping to an alternative quantification method for the pooled library tubes. The previous method (lightcycler qPCR) was accurate for weak samples but the new method (D5000 tapestation) cannot reliably quantify samples <5nM (After applying the *1.62 fudge factor in quanthub. The equivalent concentration value in Quanthub is 5/1.62 ~= 3.08642). We will change the heron clean-up to elute in a lower volume, this should increase the concentration and prevent pools <5nM being created. In the event that a pool <5nM does get quantified we need to warn the heron team that the quant method might not be perfect and they will need to perform an alternative QC before progressing. I was thinking this warning could either be added to quanthub at the point the data gets uploaded or to limber when the user scans the pool tube ready to create the normalised child tube.
Who are the primary contacts for this story
Ben F
Relates to
https://rt.sanger.ac.uk/Ticket/Display.html?id=742329
Acceptance criteria
To be considered successful the solution must allow:
- After a tapestation quant has been uploaded if a concentration is below 5nM there should be a warning about alternative QC
- ...
Dependencies
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Additional context
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Closes RT742329
UPDATE: Colour has changed to purple since this screenshot was taken.
Highlighting low conc:
When hover mouse over cell:
New design to show outliers and warnings at the same time:
