Problem to split fastq files downloaded manually in SRA

[Begoniamatters]

Environment specifications:
fastq-dump : 2.9.6
conda 24.3.0
Linux OS: "Rocky Linux 8.9 (Green Obsidian)"

I have manually downloaded fastq files from Short Reads Archives, and try to split them.
The following error appears.

gunzip SRR25402840.fastq.gz

fastq-dump --split-files SRR25402840
2024-04-26T07:45:28 fastq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -9984 ( X509 - Certificate verification failed, e.g. CRL, CA or signature check failed )
2024-04-26T07:45:28 fastq-dump.2.9.6 sys: mbedtls_ssl_get_verify_result returned 0x4008 (  !! The certificate is not correctly signed by the trusted CA  !! The certificate is signed with an unacceptable hash.  )
2024-04-26T07:45:28 fastq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.29.110' from '10.25.1.10'
2024-04-26T07:45:28 fastq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'www.ncbi.nlm.nih.gov' (130.14.29.110) from '10.25.1.10'
2024-04-26T07:45:28 fastq-dump.2.9.6 err: item not found while constructing within virtual database module - the path 'SRR25402840' cannot be opened as database or table

[Andrew Klymenko]

1. Download our latest toolkit;
2. we don't support conda.