kdillmcfarland / micro_ecol

Scripts used in the analysis of amplicon microbiota data in R

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micro_ecol

Scripts used in the analysis of amplicon microbiota data

clean_repFasta_FAST.py

Renames fasta sequences output from mothur from automatically generated name to OTU #

Pairwise_adonis

Functions to run pairwise PERMANOVAs with multiple comparison (fdr) correction across all levels variables of interest across beta-diversity measures. Adapted from Pedro Martinez Arbizu (https://www.researchgate.net/post/How_can_I_do_PerMANOVA_pairwise_contrasts_in_R).

  • Multiple variables of interest allowed.
  • Bray-Curtis, Jaccard, or UniFrac allowed.
  • Stratification of model allowed with strata option.
  • Output saved to object in R environment.

pairwise_permdisp.R

Permutational tests of beta-dispersion (PERMDISP) across multiple variables of interest and pairwise with TukeyHSD. Multiple variables of interest allowed. Bray-Curtis or Jaccard only.

R_krusk.R

Automates Kruskal-Wallis test execution on OTUs of interest as determined by simper_pretty.R. See https://github.com/asteinberger9 for up to date version

simper_pretty.R

Automates SIMAPERA execution for comparisons OTUs across variables of interest. See https://github.com/asteinberger9 for up to date version

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Scripts used in the analysis of amplicon microbiota data in R

License:GNU General Public License v3.0


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