isinaltinkaya / angsd-troubleshooting-guide

Geek Repo:Geek Repo

Github PK Tool:Github PK Tool

ANGSD Troubleshooting Guide

1. Replicate the issue using the most recent version of ANGSD

git clone https://github.com/ANGSD/angsd/; cd angsd; make

This will build angsd using the htslib submodule.

After building it, you can use realpath angsd to obtain the realpath to the angsd executable.

$ realpath angsd
/home/example/path/angsd/angsd

Use this path to replace all angsd commands in your script to make sure you are using the correct version. If you are using any utilities make sure you use the fullpath for them, too.

For example, replace

angsd -out out -doMajorMinor 1 -doMaf 3 -bam bam.filelist -GL 2
realSFS pop1.saf.idx pop2.saf.idx

with

/home/example/path/angsd/angsd -out out -doMajorMinor 1 -doMaf 3 -bam bam.filelist -GL 2
/home/example/path/angsd/misc/realSFS pop1.saf.idx pop2.saf.idx

About