TBtools is ONLY freely available for academic research. And for commercial usage, please contact me (ccj0410@gmail.com)
TBtools对于学术研究以及学术培训（由学术机构开设的公益性质且不收费的培训）是免费的。对于其商业用途，请通过我的邮箱联系我。
Use TBtools for fee-based Training is included in "Commercial usage".
商业用途包括在收费的培训中使用TBtools。
TBtools is a Toolset written by CJ-Chen for wet-lab biologists.
Up to now, it contains functions more than that listed HERE:
新增热门工具，
- Amazing Heatmap Generator热图工具
- Amazing Gene Viewer同时展示进化树，MEME结果和基因结果与结构域
序列操作工具集，Sequence Toolkits
- Fasta序列（含区段）提取，Amazing Fasta Extractor、Fasta Extractor和Fasta Subseq
- Fasta序列合并和分割，Fasta Merge and Split
- 序列格式化（序列反向、互补和按指定行宽分行），Sequence Manipulator
- NCBI序列批量下载，NCBI Seq DownLoader
- cDNA序列完成开放阅读框预测，Get Complete ORF
- 序列库中引物特异性检测，Check Primers
- gff3/gtf序列提取，Gtf/Gff3 Sequences Extractor
Blast界面化工具集，Blast
- 调用NCBI API进行远程Blast，Remote Blast
- 少量序列Blast比对到大序列库文件，Auto Blast SeveralSequences To a Big File
- 少量序列比对到少量序列，Auto Blast TwoSequence Sets
- 大序列库文件比对到大序列库文件，Auto Blast TwoSequence Sets -Big File-
- 序列比对到Fastq文件，Blast Several Seq ToFastQ
- 互惠Blast（用于不同序列库ID映射），Reciprocal Blast
- 序列比对结果区段映射图绘制，Blast XML AlignmentShower
- 序列比对结果点阵图绘制，Blast XMLDotplot
- 序列比对结果堆叠图绘制，Blast XMLPileup Grapher
- BlastXML结果转换为TBtools比对结果表格，TranFormat Blast .xml to TBtools.table
- BlastXML结果转换为Blast表格结果，TranFormat Blast .xml to Blast Table
- 基于测序数据的电子克隆，e-GenomeWalkingor e-Race
GeneOntology和KEGG工具集，GO and KEGG
- GO注释，GO Anotation
- GO富集，GO Enrichment
- GO层级统计和绘制，GO Level Counter
- GO层级比较，GO Level Compare
- GO注释解析，GO Term Parser
- GO注释转换为BiNGO输入结果，Prepare GO Annotaiton For BinGO in cytoscape
- KEGG通路富集工具，KEGG Enrichment Analysis
- KEGG****通路差异表达基因描图工具，KEGG Pathway Map Drawer
其他工具集，Others
- 取色器，Color Picker
- 表格提取（行筛选），Table IDManipulator
- 表格列操作（列排序和保留），Table ColumnManipulator
- 大文本极速查看（支持.gz压缩格式），Big Text Viewer
- 大表格极速查看（支持.gz压缩格式），Big Table Viewer
- 基于标识符号提取文本区段，Text Block Extractor
- 表达量快速查看/比较，Expression Shower
- 读段计数做RPKM/FPKM/TPM标准化，Expression Calculator (RPKM/FPKM/TPM)
- 2~6组可交互韦恩图绘制，Wonderful Venn (Up to Six Sets)
- 基于序列在染色体上展示基因位置，Map Genes OnGenome From Sequence Files
- 基于坐标在染色体上展示基因位置，Map Genes OnGenome From Position Info Files
- 基因组共线性分析结果可视化，Dual SystenyPlotter for MCscanX
About工具，About
- 默认界面（可直接拖拽.png/.jpg图片，输出Kmeans聚类色卡），About TBtools
- debug对话框，Debug Dialog
- 调整TBtools初始化内存，Resize JVMmemory
It can run in all Operating system including Mac, Linux, Windows, which have Java run time environment higher than 1.6. Both command line usages and GUI manipulation have been implemented. U can use it and distribute it freely, for researches or for commecial purpose. Well, if u found it useful to u, I will be very graceful that u cite TBtools in u paper...
- Hao Chen from Hunan Agricultural University
- Kun Lu from Southwest University