code to reproduce analyses in the manuscript
Raw data files can be obtained from the ArrayExpress under accesion E-MTAB-13874 and processed anndata objects are available from muscleageingcellatlas.org.
- jupyter notebooks for QC, integration and annotation of coarse and fine-grained cell types in the skeltal muscle.
- scripts for running single-cell linear mixed effect model (R-based) to evaluate differential gene expression with age accounting for biological and technical covariates (see Supplementary Note 1.3 in this study for detailed explanation of this implementation).
- jupyter notebooks to run analyses to reproduce main and extended data figures.
TBA