Command line application to get reverse barcodes and their frequencies in genome sequences.
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Run barcode_reverse_pair.py in Linux Terminal
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You will be given three user input prompts:
"Enter path to .fastq file:" (.fasta can also be used) "Enter start position of sequence:"
"Enter stop position of sequence:" -
The terminal will output the time in seconds (s) it took for the program to execute and the memory usage of the program in megabytes (MB):
"Program execution time = * s"
"Memory usage of program = * MB" -
Two CSV files will be written to the wkdir:
- paired_barcodes_revcomps.csv : Barcode and Reverse Barcode pairs and their frequency counts.
- unpaired_barcodes.csv. : Unpaired barcodes and their frequency counts.