PhyloCut is a program to cut large multigenic families at duplication nodes. It works with gene trees annotated with species IDs and duplication/speciation node annotations. Command: phylocut input.sequence.file=XX.fasta input.tree.file=XX.tree ids.to.cut=XX.id As you can guess, phylocut takes an alignment (e.g. fasta format), a corresponding gene tree in NHX format, as produced by Phyldog, and a file containing a list of species IDs. These species IDs identify the duplications at which the gene tree should be cut. The format is one id (one number) per line.