AHdeRojas / masame

Code to run the shiny functions for the MASAME suite

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The Multivariate AnalysiS Applications for Microbial Ecology (MASAME)

The apps in this directory will allow you to perform a set of statistical analysis and screens typically suited to ecological data sets. These accompany an online guide to statistical analysis in microbial ecology, available here: http://mb3is.megx.net/gustame

Please see the following publication for more: Buttigieg PL, Ramette A (2014) A Guide to Statistical Analysis in Microbial Ecology: a community-focused, living review of multivariate data analyses. FEMS Microbiol Ecol. 90: 543–550. http://dx.doi.org/10.1111/1574-6941.12437

If you have queries, wish to report errors, or have enhancement requests, contact Pier Luigi Buttigieg.

Please fork and submit pull requests!

If you'd like to fork the code to improve or extend it, we're more than happy to integrate pull requests here and in GUSTA ME. It would be even better if you have knowledge to share via GUSTA ME itself! Of course, all contributions will be micro-credited.

Analysis Apps

Running apps locally

You can run any of these apps on your own machine (assuming you've cloned the code locally and have all the R packages you need) by using the runApp() function from shiny. These are at the top of most scripts in this repo. For example, to run the ANOSIM app on your computer from the top level directory ("masame"), type the following in your R console:

runApp('./ANOSIM', launch.browser = TRUE) 

Running apps online

To launch an analysis app online, please click the link to the corresponding GUSTA ME page below and scroll to the bottom where the a launch link will be provided.

Data prep

Ordination & clustering methods

Hypothesis testing

Example data sets

Please find demo data sets in the "Example data sets" directory. These are correctly formatted for upload to MASAME Apps under the default upload settings. More information is available in the README file in the directory.

Experimental updates

Some apps have been updated to support colour-coding, vegan's ordination hulls, spiders, and ellipses, and more transformations. If you'd like to try them out, they're in the experimentalUpdates folder. You'll have to run them locally (see below for brief instructions), but it's quite straightforward to do so.

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Code to run the shiny functions for the MASAME suite


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