Nanopore analysis of CO-transcriptional processing (nano-COP) exposes the nascent transcriptome via stringent purification of nascent RNAs and direct long-read RNA-sequencing through nanopores. Nano-COP reveals the physical distance between transcription and splicing as well as splicing order and coordination across multi-intron transcripts.
This repository includes the scripts and annotation files needed to analyze nano-COP data. The "annotation_files" folder contains gene feature coordinates from human and Drosophila datasets that are utilized for analyzing nano-COP datasets. The "scripts" folder includes the python code to make necessary datasets and plots for each figure in the manuscript.
Here is the link to our publication in Molecular Cell for more details on the nano-COP method: https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30865-2