This IPython notebook provides step-by-step analysis of qPCR data for quantitation of NGS libraries.
To install IPython, run:
$ easy_install ipython[all]
Note: this (and IPython itself) requires setuptools installed.
Other dependencies needed:
$ pip install plotly
$ pip install numpy
$ pip install pandas
You will also need a plotly account to generate graphs. This can be setup at https://plot.ly
To run the notebook, open a terminal and type:
$ ipython notebook