What is the reason for a difference in arm length between ligation and extension arms?

Question

What is the reason for a difference in arm length between ligation and extension arms?

RoyBSimons opened this issue a year ago · comments

Dear shendurelab,

I noticed that the ligation arm and extension arms are of different length.
Is there a reason to let the ligation arm bind earlier than the extension arm?
In various MIP-studies the arms are of equal size.

Kind regards,
Roy

Evan Boyle · Answer 1 · Tue Jul 18 2023 22:30:29 GMT+0800 (China Standard Time)

Hello,You can review the article to learn about optimal arm length: supplementary figure 3 shows that longer ligation arms relative to extension arms perform better! https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155255/bin/supp_btu353_bioinformatics_supplemental4.docEvanCourtesy of my phoneOn Jul 18, 2023, at 1:45 AM, Roy Simons ***@***.***> wrote: Dear shendurelab, I noticed that the ligation arm and extension arms are of different length. Is there a reason to let the ligation arm bind earlier than the extension arm? In various MIP-studies the arms are of equal size. Kind regards, Roy —Reply to this email directly, view it on GitHub, or unsubscribe.You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>