ryananeff / mycbp2_mutational

Code for the project on MYCBP2 dysregulation in human breast cancers

MycBP2 in DNA-damage induced apoptosis experiments

Authors: Ryan A. Neff, Almudena Bosch-Gutierrez , Yifei Sun , Igor Katsyv , Won-min Song, Minghui Wang, Martin J. Walsh, and Bin Zhang

Contributed equally to the work

This repository includes code for the manuscript Dysfunction of Ubiquitin Protein Ligase MYCBP2 Leads to Cell Resilience in Human Breast Cancers. This includes three sets of experiments

1. Analyses performed on TCGA-BRCA data leading to the discovery of MYCBP2 perturbations
2. Flow cytometry of apoptosis and cell cycle experiments in MCF10A cells treated with MycBP2 siRNA (knockdown) and with or without cisplatin, a DNA-damage inducing agent
3. Bulk RNA-sequencing of MCF10A cells treated with MycBP2 siRNA (knockdown) and with or without cisplatin

All correspondence should be sent to: Bin Zhang <bin.zhang at mssm.edu>, Center for Transformative Disease Modeling, Icahn School of Medicine at Mount Sinai, New York, NY 10029

A repository which includes data and analyses files for these experiements can be found at http://dx.doi.org/10.7303/syn25650387.