Possible bug in snaptools dex-fastq, on snATAC dataseq generated with latest 10X snATAC V1.1 chemistry
Qi-Yang-Bioinfo opened this issue · comments
I was running SnapATAC on a dataset generated with latest 10X snATAC V1.1 chemistry, from scratch(Not on cell ranger results).
It seems that snaptools dex-fastq is not aware of the change of barcode position, thus grabbed the wrong part and treated as barcode.
I was running SnapATAC R and No common barcode exist between snapobject@barcodes and 10X_singlecell_file@barcodes, wired barcode sequence shown in snapobject@barcodes like AAAAAAAAAAAAAAAA(shouldn't exist) made me believe this is a bug in snaptools dex-fastq