ZebrafishBMD: A software program for benchmark dose calculations using toxicity screening data from high-throughput zebrafish assays
The ZebrafishBMD program was written by Dr. Dennis Thomas and Dr. Harish Shankaran in collaboration with Dr. Katrina Waters, at the Pacific Northwest National Laboratory. The program was developed as part of projects funded by the National Institutes of Health and the Environmental Protection Agency.
The program calculates BMD10 values for 22 developmental and 2 behavioral endpoints of zebrafish using the methods described in the paper.
The current version (ZebrafishBMD v1.0) is a Windows standalone application that was created using Matlab 2016b.
Matlab Runtime v. 9.1 (Matlab 2016b) for Windows
-
Download and unzip the following .zip folder files
ZebrafishBMD_v1.0.zip
example_data_v1.0.zip
-
Move the
input_data
folder fromexample_data_v1.0
intoZebrafishBMD_v1.0
folderThe
input_data
folder contains zebrafish developmental and behavioral assay data for 10 chemicals. Details about the assay and computational approach are provided in this paper. -
Go to
ZebrafishBMD_v1.0
folder on Windows command prompt window -
Type
ZebrafishBMD.exe
and press Enter. A user interface window will open. The text fields are pre-filled, so the users don't have to edit these fields. Simple click on the 'Run' button at each step after the previous step run is completed. -
The program will create the following folders to save the run output files:
- morphology_response_data
- morphology_fit_results
- behavior_response_data
- behavior_fit_results
Please cite
Thomas, D. G., Shankaran, H., Truong, L., Tanguay, R. L., & Waters, K. M. (2018). Time-dependent behavioral
data from zebrafish reveals novel signatures of chemical toxicity using point of
departure analysis. Computational Toxicology. https://doi.org/10.1016/j.comtox.2018.11.001
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