orion-cirb / Isolectin_Dextran

Isolectin_Dextran

• Developed for: Iris
• Team: Germain
• Date: May 2023
• Software: Fiji

Images description

2D images of sections of mouse heart taken with a x20 objective.

2 channels:

1. CSU_561: Dextran
2. CSU_635: Isolectin

Macro description

• Detect vessels in isolectin channel with Gaussian blur filtering + Huang thresholding
• Fill in dark isolectin mask in dextran channel
• Detect extravasated dextran in dextran channel with Gaussian blur filtering + Otsu thresholding
• Give isolectin and extravasated dextran masks areas

Version history

Version 1 released on May 30, 2023.