tombo resquiggle 0%|

[Li-Rui091]

Hi，
I run resquiggle on my test data ,the command as follows:
thread=2
params_fast5=/public211/lirui/m6Adection/data/GCP1/test
params_ref=/public211/lirui/m6Adection/data/reference/Arabidopsis_thaliana.TAIR10.cdna.all.fa
tombo resquiggle --rna --processes ${thread} --corrected-group RawGenomeCorrected_000 --basecall-group Basecall_1D_000
--include-event-stdev --overwrite --ignore-read-locks ${params_fast5} ${params_ref}

However, the log shows that the process has been 0% for more than 24 hours：
[17:41:53] Loading minimap2 reference.
[17:42:02] Getting file list.
[17:42:02] Re-squiggling reads (raw signal to genomic sequence alignment).
0%| | 0/2 [00:00<?, ?it/s]

Any advice for me to fix it is appreciated.

[Kennedi Todd]

@Li-Rui091
This happened to me and I realized I didn't have the hdf5 plugin. Check to see if you have it already by typing
h5repack. If the usage is displayed then you have it. If not, follow the install instructions below. This is installation instructions for Linux.

wget https://github.com/nanoporetech/vbz_compression/releases/download/v1.0.1/ont-vbz-hdf-plugin-1.0.1-Linux-x86_64.tar.gz
tar xvzf ont-vbz-hdf-plugin-1.0.1-Linux-x86_64.tar.gz
export HDF5_PLUGIN_PATH=/tools/ont-vbz-hdf-plugin-1.0.1-Linux/usr/local/hdf5/lib/plugin
h5repack

[keenhl]

I have the same issue of resquiggle being stuck at 0%. I checked hdf5 and do have it installed. Any other suggestions to get tombo resquiggle to work? Thanks.