Different output in wig file in every running

[kwonej0617]

Hello, developer.

I have a question regarding wig file.
I was wondering if there could be a case where the output contents of wig files are different in every run.
For example, I run Tombo with fast5 public data. The fast5 data were contained in different subf-olders and each folder has 4000 single-format fast5 files.
Using fast5 files in each sub-folder, I run Tombo, then checked whether .wig and .bedgraph files are successfully generated. However, I found that in a sub-folder, plus.wig was successfully generated but minus.wig file had only a head without contents. So I run Tombo again, and minus.wig were not generated again. Also, I found the contents in plus.wig were different from the previous one.

In my first running, the contents of plus.wig were, 
variableStep chrom=chr6 span=1
1395673 0.0000
1395675 0.0000
1395676 1.0000

In the second running, the contents of plus.wig were, 
variableStep chrom=chr2 span=1
278101 0.0000
278102 0.0000
278103 0.0000

1. I wonder why I got different results between each run.
2. Also, would be any specific reason that minus.wig are not generated?

Any advice would be appreciated.
Thank you.

[kwonej0617]

It seems that only the order of content is changed.