nanoporetech / rerio

Research release basecalling models and configurations

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Urgent Help Required: Regarding Error for modified basecalling

PRIYANKA-22091995 opened this issue · comments

I was trying to run the following command for modified basecalling:
guppy_basecaller -i /DATA/BioLab/Priyanka/FAST5/EB_EL_FAST5/fast5/ -s /DATA/BioLab/Priyanka/FAST5/ -c /home/arnav/workspace/rerio/basecall_models/res_dna_r941_min_modbases-all-context_v001.cfg --recursive -a /home/arnav/workspace/PlasmoDB-61_PvivaxSal1_Genome.fasta --bam_out -x cuda:0 -m /home/arnav/workspace/rerio/basecall_models/res_dna_r941_min_modbases-all-context_v001.jsn --barcode_kits EXP-NBD104

But got the below error.
guppy error

Hi @PRIYANKA-22091995,

It looks like you're using guppy-v6.3.2 here, however this model lists compatibility with guppy-v3.5.1 (README) and it seems like this is causing an issue with model loading. Are you able to retry with an older guppy version?