nanoporetech / bonito

A PyTorch Basecaller for Oxford Nanopore Reads

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5mC detection for RNA

keenhl opened this issue · comments

We have fast5 files generating using the RNA002 chemistry. We are interested in determining the methylation status (5mc) of these reads.

How do we proceed to do this Bonito? Alternatively, if this is not something Bonito can do, what application would be recommended?

Thanks for your help.