mjpdejong / unanimity

Consensus library and applications

Unanimity

C++ library and its applications to generate and process accurate consensus sequences

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Documentation

• Getting Started
• Projects
  • Available
    • Consensus Core
    • Circular Consensus Calling ccs
    • Minor Variant Calling juliet
    • Reduce Alignment fuse
    • Swap Alignment Reference cleric
    • Minor Variant Pipeline julietflow
  • Planned
    • Genomic Consensus Calling gcpp
    • Viral Haplotype Phasing eden
• Developer environment
• PacBio open source license

Quick Tools Overview

Circular Consensus Calling

ccs takes multiple reads of the same SMRTbell sequence and combines them, employing a statistical model, to produce one high quality consensus sequence.

Genomic Consensus Calling

gcpp will replace the current python GenomicConsensus, until then please use the existing solution.

Minor variant caller

juliet identifies minor variants from aligned ccs reads.

Reduce alignment

fuse reduces an alignment into its closest representative sequence.

Swap BAM reference

cleric swaps the reference of an alignment by transitive alignment.

Minor variant pipeline

julietflow automatizes the minor variant pipeline.

Viral Haplotype Phasing

eden will leverage CCS reads to identify low-frequency haplotypes within polyploid samples.

Help

Support is only provided for official and stable SMRT Analysis builds provided by PacBio and not for source builds.