mikailweston/example

The KlustaSuite is an open source spike sorting software suite designed for extracellular recordings obtained with large multielelectrode arrays (with hundreds of channels).

The KlustaSuite contains three programs:

SpikeDetekt for detecting and extracting spikes from raw recordings (written in Python).
KlustaKwik for automatically clustering the detected spikes (written in C++).
KlustaViewa for manually processing the results of the automatic step (written in Python).

It is developed in the Cortical Processing Laboratory at UCL by:

Cyrille Rossant (KlustaViewa)
Shabnam Kadir (SpikeDetekt and KlustaKwik, firstname at cortexlab.net)
Dan Goodman
Max Hunter

Please send feedback to Kenneth Harris (firstname at cortexlab.net).

Subscribe to our googlegroups by sending an email to: klustaviewas+subscribe 'at' googlegroups.com

Repositories

Here are two public Google Drive repositories where we store our software installers and example datasets.

Installation instructions

Make sure you have access to the two repositories (see above).

The installation instructions differ according to your operating system.

Windows users

Download and run the all-in-one installer for Windows 64-bit klustaviewa-setup.exe here. You may need to restart your computer afterwards to ensure that your system PATH is updated.
Download the example package (150MB) klusta-example.zip in the example data repository and extract it in a directory.
Open a terminal and go to that directory.
Run the automatic spike detection and clustering (~15 min running time) by typing in the console:
```
 klusta params.prm
```
The PRM file is a text file (written in Python) containing all parameters the KlustaSuite requires in order to spike sort the data. You will see a PRB file too: this text file (written in Python) contains information about the probe. See below for more details.
Then, double-click on the KlustaViewa icon on your desktop and do File > Open and select test_hybrid_120sec.kwik.
To update the program, download klustaviewa<...>.exe in the software repository.

Linux and Mac OS X

Download and install a Python 2.7 distribution (see further instructions here).
Download and extract the KlustaViewa ZIP package here (GREEN .ZIP BUTTON).
Open a terminal and type:
```
 python setup.py install
```
Install klustakwik and put its location in your system PATH.
Download the example package (150MB) klusta-example.zip in the example data repository and extract it in a directory.
Run the automatic spike detection and clustering (~15 min running time) by typing in the terminal (you need to be in the directory where you extracted the example package):
```
 klusta params.prm
```
The PRM file is a text file (written in Python) containing all parameters the KlustaSuite requires in order to spike sort the data. You will see a PRB file too: this text file (written in Python) contains information about the probe. See below for more details.
Run KlustaViewa to open the processed dataset and proceed to the manual step:
```
 klustaviewa
```
In KlustaViewa, do File > Open and select test_hybrid_120sec.kwik.
To update the program, perform 2 and 3 again.

Overview of the Kwik format

Our software suite currently accepts a raw data file in .dat format or .raw.kwd format. It creates a set of files in the Kwik format.

Let's say you give the name 20140606-007-experiment to your experiment. The data will be stored in the following files:

20140606-007-experiment.kwik
20140606-007-experiment.kwx
20140606-007-experiment.raw.kwd
20140606-007-experiment.low.kwd

These files contains data from all shanks.

The kwik file contains all metadata, the spike times (previously .res), the clusters (previously .clu). It is a relatively small file (a few MB in general).
The kwx file contains the features (previously .fet), the masks (previously .fmask), the waveforms (previously .spk).
The raw.kwd file contains the raw recordings.
The low.kwd file contains the low-pass filtered recordings (LFP).

The kwik file contains all scientific information you need in general (spike times and clusters). The kwx file contains large volumes of data related to spike sorting (features and waveforms). The kwd files contains raw data.

For spike sorting, you can discard the kwd files.

You can open these files in MATLAB or any other language with HDF5 support (in Python, with h5py or PyTables). You can export to the old Klusters format with kwikkonvert.

Information about the PRM file

The parameter file (with a name something like myexperiment.PRM) contains further information about how to detect spikes. See the default PRM file here.

More details here.

Information about the PRB file

See here.

Details on the test dataset

2 minutes recording, 32 channels at 20kHz, 150MB.

How to debug SpikeDetekt

If SpikeDetekt is giving strange results, run the diagnostics module to visualize the problem. To do so:

Download the diagnostics module in this repository and save it near your PRM file

In your PRM file, add the following:

diagnostics_path = '/path/to/diagnostics.py'  # path to the diagnostics module
diagnostics_time_samples = [123, 456]  # put here the time samples of the spikes you want to debug

See the test_diagparams file for details of more variables that can be set for this particular diagnostic module.

Run SpikeDetekt as usual.

Here is how it works: this diagnostics function will be called in the main loop and all local variables are passed to the function.

You can use your own diagnostics function: just make sure to use def diagnostics(..., **extra_params) so that extra local variables are silently passed to the function. Look at the main loop's code to find out which local variables you have access to.

You can also drop in IPython by putting this in your diagnostics function: from IPython import embed; embed().

Contact

If you have any trouble, bug, comment or suggestion:

You can send a message on the Google group.
You can send us an e-mail.

How to cite

If you have used KlustaKwik for a scientific publication, please cite our paper, 'High-dimensional cluster analysis with the Masked EM Algorithm' by Shabnam N. Kadir, Dan F.M. Goodman and Kenneth D. Harris (2014) Neural Computation, 26:2379-2394.

If you have used SpikeDetekt and/or KlustaViewa, please cite "Spike sorting for large, dense electrode arrays" by Cyrille Rossant*, Shabnam N Kadir*, Dan F. M. Goodman, John Schulman, Mariano Belluscio, Gyorgy Buzsaki, Kenneth D. Harris (2015). bioRxiv http://dx.doi.org/10.1101/015198. * joint contribution.

mikailweston / example