Authors: Taejoon Kwon (tkwon@unist.ac.kr)
- PhageM13_genome.V00504.fa - from https://www.ebi.ac.uk/ena/browser/view/V00604.2
- PhageM13_genome.JX412914.fa - from https://www.ebi.ac.uk/ena/browser/view/JX412914.1
- Green: a variable region (12 amino acids == 36 nucleotides)
- Red: a target of PhageM13_ILMN_F
- Orange: a target of PhageM13_ILMN_R
- Yellow/Cyan : a pair of reads with 2x150bp sequencing (a bold hexamer will be dropped due to the UMI).
- Nextera_i5, Nextera_i7 - a pair of conventional Illumina Nextera barcode adapters.
- PhageM13_ILMN_F & PhageM13_ILMN_R - a pair of primers for phage M13 variable region amplification.
- 'NNNNNN' - a hexamer used for UMI (to estimate the amplification bias)
- ipcress (for in silico PCR): https://www.ebi.ac.uk/about/vertebrate-genomics/software/ipcress-manual