latchbio / wf-guide_counter

A better, faster way to count guides in CRISPR screens. Latch wrapper of tool by Fulcrum Genomics.

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guide-counter hosted with the Latch SDK

This is an implementation of the guide-counter NGS count generation tool with Latch SDK.

guide-counter is a tool for processing FASTQ files from CRISPR screen experiments to generate a matrix of per-sample guide counts. It can be used as a faster, more accurate, drop in replacement for mageck count. By default guide-counter will look for guide seqeunces in the reads with 0 or 1 mismatches vs. the expected guides, but can be run in exact matching mode.

Uploading your own guide-counter

The contents of this repository were modified from the boilerplate provided by latch init.

You can create your own workflow by

You can replicate the contents of this repo by installing latch and creating some boilerplate like so:

$ pip install latch
$ latch init wf-guide_counter
$ cd wf-guide_counter

You will notice the structure of your boilerplate repository is roughly the same as this repository. Just modify the code in the wf/__init__.py and the Dockerfile, run latch register and you should be able to upload your own version of guide-counter.

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A better, faster way to count guides in CRISPR screens. Latch wrapper of tool by Fulcrum Genomics.


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