jttkim / kewbioinfo

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Post HybSeq Workshop Session(s)

  • Illumina NGS basics (firm up what clusters and PHRED scores are)
  • basic Linux commands, command line structure
  • FastQC recap
  • PHRED scores and their encoding (aka what is phred33?)
  • File permissions (aka why are the EGL*.fastq files and the NZ*.fastq files shown in different colours?)
  • Intro into bash program control flow and I/O redirection (aka how does that while read name do reads_first.py ... done < namelist.txt thing work?)
  • alignments, multiple alignments
  • processes (and threads), multiprocessing (aka what's this GNU parallel thing?)
  • principles / heuristics / tips and tricks / hacks ... and how to distinguish these

Recommended Resources


Please send suggestions to me at jttkim@gmail.com or j.kim@kew.org

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