How do bases and segments correspond one to one?
N-damo opened this issue · comments
I'm not sure what you mean. A segment, which I think is now commonly referred to as an "event" in the literature, is a slice of signal that exhibits steady statistical parameters. In theory, each segment should correspond to a change of one nucleotide in the pore.
A nanopore can hold k nucleotides (k-mer)
simultaneously. For example, k equals 5 for the pore version
R9.4. Thus, the current changes indicate the k-mers that pass
through the nanopores. I'm not sure whether each segment correspond to one nucleotide here.
Yes, in theory a segment should correspond to one nucleotide change in the pore. In this library, it's just a way to store a chunk of signal with stationary statistical properties. Whether the segments actually correspond to changes in the pore depends on the quality of the segmentation algorithm.
everything is amazing. In the supervised machine learning, back to the very beginning, people how to know raw signal correspond to a real sequence.