# SPEKcheck SPEKcheck is a web application to visualise the efficiency of the light path in a fluorescence microscope. It can run locally and completely offline, or it can be configured to run in a site. SPEKcheck models an optical setup as a detector, a dye, an excitation source, and an arbitrary number of filters in both the excitation and emission paths. SPEKcheck then reports values for the excitation efficiency of the dye, the collection efficiency of the emitted fluorescence, and a 'brightness' score, all easy comparison between different fluorescent labels. It also displays a spectral plot of various components, and the final emitted fluorescence. ## Trying SPEKcheck A public of instance is available online at https://www.micron.ox.ac.uk/software/spekcheck ## Documentation A manual with configuration and installation options is included in the release. It is also available online at https://www.micron.ox.ac.uk/software/spekcheck/help.html ## Installation 1. Download the latest release from the releases page: https://github.com/MicronOxford/SpekCheck/releases/ 2. SPEkcheck can then run in two modes: 1. To run without a web server, simply open the `index.html`. Browsers other than Firefox will require extra configuration to run it this way, see `help.html` for details. 2. To run with a web server, move the SPEKcheck directory into whatever directory your web server is configured to serve. Alternatively, run `python -m SimpleHTTPServer` and then connect to http://127.0.0.1:8000/index.html