Data and scripts to model eIF3 from MS data.
The method used here is the same as that used for the MS benchmark.
To generate models, run the ms_cg.py script. This will generate a number of models of the complex, named conf_eif3.<num>.pym.
The .pym files can be converted to .tcl files by running the pym2tcl.py script, and then to .mfj files by running the tcl2mfj.py script.
Author(s): Argyris Politis, email:argyris.politis@kcl.ac.uk
Version: 1.0
License: LGPL. This library is free software; you can redistribute it and/or modify it under the terms of the GNU Lesser General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version.
Last known good IMP version:
Testable: Yes.
Parallelizeable: No
Publications:
- Keren Lasker, Javier A. Velazquez-Muriel, Benjamin M. Webb, Zheng Yang, Thomas E. Ferrin, Andrej Sali, Macromolecular assembly structures by comparative modeling and electron microscopy, Methods in Molecular Biology, 2011.
- Politis A, Stengel F, Hall Z, Hernandez H, Leitner A, Waltzhoeni T, Robinson CV, Aebersold R. A mass spectrometry-based hybrid method for structural modelling of protein complexes, Nature Methods, 11, 403-406, (2014)
- A. Politis, C. Schmidt, E. Tjioe, A. Sandercock, K. Lasker, Y. Gordiyenko, D. Russel, A. Sali, C. Robinson. Topological models of heteromeric protein assemblies from mass spectrometry: Application to the yeast eIF3:eIF5 complex, Chem Biol 22, 117-128, 2015.