Jupyter notebook used for analyzing Calcium propgation velocities from high-speed video microscopy data. Analysis of aligned cardiac microtissues, cultured in a Heart-on-Chip shown. Extracted profiles are used to compose Fig. S2 in Schneider et al. (2022).
Determine the signal onset time for each x-position along the muscle fiber. From the propagation along the fiber, the Calcium velocity is determined.
Video of fluorescence intensity, recorded at ~ 200 fps. Frames are saved as series of .tif-files in each folder.
Analysis in notebook produces following plots indicating the color-coded signal propagation and corresponding fit to determine the propagation velocity:
Please cite O. Schneider, A. Moruzzi, S. Fuchs, A. Grobel, H. S. Schulze, T. Mayr, and P. Loskill, Fusing spheroids to aligned μ-tissues in a heart-on-chip featuring oxygen sensing and electrical pacing capabilities, Materials Today Bio 15, 100280 (2022) if you reuse some of the code for your own analysis.