Real-time quantitative PCR (qPCR) is a broadly used technique in the biomedical research. Currently, few different analysis models are used to determine the quality of data and to quantify the mRNA level across the experimental conditions.
By the end of this course, participants will be able to:
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Implement methods for quality assessment, analysis and testing qPCR data for statistical significance.
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Understand Double Delta CT and standard curve models were implemented to quantify the relative expression of target genes from CT in standard qPCR control-group experiments
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Calculation of amplification efficiency and curves from serial dilution qPCR experiments are used to assess the quality of the data.
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Two-group testing and linear models were used to test for significance of the difference in expression control groups and conditions of interest.
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