hcstubbe / adv_infection

Analyses scripts for the E2A/p53 infection manuscript

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E2A/p53 infection manuscript

Code summary

This repository contains the analyses scripts for the E2A/p53 infection manuscript:

  • A1-A2: Illumina HiSeq4000 data were analyzed using Kallisto and Sleuth.

  • B1-B2: Oxford Nanopore PromethION data were basecalled with Guppy in a Docker/enroot container. The samples were demultiplexed using pytrim2 on a Docker/Charliecloud container. The folders B1 and B2 contain the relevant scripts and Docker files. The containers were run on the LRZ Linux cluster cloud

  • Demultiplexed ONT full-length reads were analyzed for differential gene expression using Oxford Nanopore's DEG transcriptome pipeline.

  • C1: Gene expression data from Illumina and PromethION were integrated and analyzed.

Data availability

The Illumina and PromethION data were submitted to GEO.

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Analyses scripts for the E2A/p53 infection manuscript


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