gerbenvoshol / primer3

Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).

Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl.

Installing

sudo apt-get install -y build-essential g++ cmake git-all

git clone https://github.com/primer3-org/primer3.git primer3

cd primer3/src

make

make test

Run Primer3

./primer3_core ../example

Read the complete Primer3 manual

Primer3 Manual

or see /src/primer3_manual.htm