fanglab / nanodisco

nanodisco: a toolbox for discovering and exploiting multiple types of DNA methylation from individual bacteria and microbiomes using nanopore sequencing.

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nanodisco difference: Problem with `mutate()` column `N_wga`.

BioRB opened this issue · comments

Dear Developers, I m performing nanodisco difference and I get this error:

nanodisco difference -nj 2 -nc 2 -p 2 -i ~/analysis/preprocessed_subset -o ~/analysis/difference_subset -w barcode08 -n barcode03 -r ~/reference/GCF_000054005.1_ASM5400v1_genomic.fasta

Computers / CPU cores / Max jobs to run
1:local / 8 / 2

Computer:jobs running/jobs completed/%of started jobs/Average seconds to complete
local:2/0/100%/0.0s Error in { : 
  task 1 failed - "task 1822 failed - "Problem with `mutate()` column `N_wga`.
ℹ `N_wga = length(unlist(wga))`.
✖ object 'wga' not found
ℹ The error occurred in group 1: contig = NC_007618.1, position = 1822, dir = fwd, strand = t.""
Calls: %do% -> <Anonymous>
Execution halted
local:2/1/100%/576.0s Error in { : 
  task 1 failed - "task 1423 failed - "Problem with `mutate()` column `N_wga`.
ℹ `N_wga = length(unlist(wga))`.
✖ object 'wga' not found
ℹ The error occurred in group 1: contig = NC_007618.1, position = 11423, dir = fwd, strand = t.""
Calls: %do% -> <Anonymous>
Execution halted
local:2/2/100%/576.5s Error in { : 
  task 1 failed - "task 19 failed - "Problem with `mutate()` column `N_wga`.
ℹ `N_wga = length(unlist(wga))`.
✖ object 'wga' not found
ℹ The error occurred in group 1: contig = NC_007618.1, position = 20019, dir = fwd, strand = t.""
Calls: %do% -> <Anonymous>
Execution halted

thanks for your kind support.
Best RB

commented

Hello @BioRB,

Could you share the command performed prior to this step?

Thank you,

Alan