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dieterich-lab / DCC

DCC uses output from the STAR read mapper to systematically detect back-splice junctions in next-generation sequencing data. DCC applies a series of filters and integrates data across replicate sets to arrive at a precise list of circRNA candidates.

Home Page:https://dieterichlab.org/software/

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ERROR in DCC running: chimeric.out.juntcion probably corrupt

fenguita opened this issue · comments

commented

Hi

I am trying to run DCC over a pre-aligned bunch of samples. When the script starts I got an annoying error complaining about the features present in my chimeric.out.junction files:

WARNING: File /Chimeric.out.junction, line 67891 does not contain all features.
WARNING: Chimeric.out.junction is probably corrupt.

When I looked at the chimeric.out.junction file, the line that the software indicates is the last line of the file. What are the features that DCC is looking at?... Please let me know what I am doing wrong.

Best

commented

Hi @fenguita,

could you either paste the specified line plus a few extra lines around or even better upload the whole file here?

Cheers,
Tobias