This deep mutational scan of human adenosine deaminase is in the early planning phase. Currently
- have synthesized a gene and cloned into vector
- expressed and purified, and optimized purification, in Escherichia
- developed low-cost, high-throughput manual assay
- developed low-cost, high-throughput automated assay for Transcriptic (now defunct)
Next steps
- computational deep mutation scan
- feature generation
- machine learning
- error prone PCR protocol
- nicking/Kunkel/NNK oligo design and protocol
- Neat sequencing