This workflow detects spots in a 3D image by filtering the image by Laplacian of Gaussian (user defined radius) and detecting local intensity minima in 3x3x3 regions below a minimum intensity value (user defined threshold).
3D spot detection workflow
This workflow detects spots in a 3D image by filtering the image by Laplacian of Gaussian (user defined radius) and detecting local intensity minima in 3x3x3 regions below a minimum intensity value (user defined threshold).
3D spot detection workflow