This script can correct drift of supreresolution image analyzed by rapidSTORM. 1 crop fiducial image from original image (for example, using ImageJ's crop) 2 perform rapidSTORM analysis a) choose user level to Intermediate b) Input minimum spot distance value to the size of the image in order to avoid multiple particle detection in fiducial image. c) In output options, choose "Localization file" from side menu and check "Output only Malk fields (x,y,z,t,I) 3 perform rapidSTORM for whole image. Localization file should be extracted. 4 Run this macro on ImageJ a) Choose localization file for whole image b) In dialog, input parameters c) Load fiducial localization file whne you checked "Do drift correction" 5 Superresolution images will be reconstruced. Each pixel value contains each particle counts. 2013.08.06 Yoshiyuki Arai