Code related to An integrated multi-omic single cell atlas of human B cell identity: https://www.cell.com/immunity/fulltext/S1074-7613(20)30268-5
These scripts allow processing of fcs files and generation of the main figures from the manuscript. Instructions for each script are written in script headers.
screen_preprocess.R processes fcs files from the surface screen. Files can be found here: https://flowrepository.org/id/FR-FCM-Z2MA Requires: fcs files screen_metadata.scv
other_preprocess.R process fcs files from the other datasets in the manuscript. Files can be found here: https://flowrepository.org/id/FR-FCM-Z2MC Requires: fcs files figure_4_metadata.csv figure_5_biosynthesis_metadata.csv figure_5_metabolism_metadata.csv figure_5_signaling_metadata.csv figure_6_metadata.csv
screen_figures.R generates subfigures from figures 1-3. Requires: go_parsed.csv
figure_4.R figure_5.R figure_6.R generate subfigures from figures 4-6.