github-data 17 testis single cell R analysis processing all coded in this study were based on Seurat R package, see https://satijalab.org/seurat/v3.2/pbmc3k_tutorial.html the monocle analysis was based on http://cole-trapnell-lab.github.io/monocle-release/docs_mobile/ 17 testicular single cells data were first merged into one seurat and then underwent further analysis. for more information, please contact zlyinwangyi@163.com.