Socrates is an R package for analyzing Single-Cell Assay for Transposase Accessible Chromatin Sequencing (scATAC-seq) data. Socrates takes as input 1. barcode meta data and 2. a cell x feature sparse matrix in triplet format (see example data in inst/extdata). The main contribution of Socrates compared to previously established methods is a regularized quasi-binomial logistic regression for single-cell chromatin accessibility for normalizing accessibility profiles across peaks and cells.
Coming updates to Socrates will provides tool for several processing and analytical steps:
- Clustering
- Batch effect removal
- Cell-type annotation
- Co-accessibility
- Motif analysis
- Gene accessibility
- Pseudotime
- scRNA-seq integration
and much more!
All users need to begin is a counts matrix (binarized) in triplet format, or a BED file (Columns 1-3: locations of Tn5 insertions, Column 4: barcode, and Column 5 strand) with genome annotation data (gff3/gtf and fai/chromosome.sizes).
If you use Socates in your own study, please consider citing the following article:
Alexandre P. Marand, Zongliang Chen, Andrea Gallavotti, Robert J. Schmitz. (2021). A cis-regulatory atlas in maize at single-cell resolution. Cell, doi:10.1016/j.cell.2021.04.014
Current release: 03/16/21 BETA v0.0.9
Socates requires R v4.0.0 or greater.
# download the devtools package if not currently installed
install.packages("devtools")
library(devtools)
# install
devtools::install_github("plantformatics/Socrates", ref="main")
1. Loading data, quality control, identifying cells, and creating a Socrates object
2. Loading pre-processed data, dimensionality reduction, and clustering
3. Comparison of normalization methods
4. Identify co-accessible ACRs
... more coming soon