Segment nuclei in 2D images by Laplacian of Gaussian (user defined radius) and thresholding (user defined level) followed by binary watershed transform.
This workflow is described in www.biii.eu in http://biii.eu/nuclei-segmentation-2d-imagej
BIAFLOWS workflow to segment clustered nuclei using a laplacian filter, thresholding and a binary watershed transform.
Segment nuclei in 2D images by Laplacian of Gaussian (user defined radius) and thresholding (user defined level) followed by binary watershed transform.
This workflow is described in www.biii.eu in http://biii.eu/nuclei-segmentation-2d-imagej
BIAFLOWS workflow to segment clustered nuclei using a laplacian filter, thresholding and a binary watershed transform.